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primary antibodies against cd45  (Novus Biologicals)


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    Novus Biologicals primary antibodies against cd45
    Primary Antibodies Against Cd45, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+cd45/cd45+antibody/10__1158_slash_2159___8290__cd___24___1208-373-0-6
    Average 90 stars, based on 1 article reviews
    primary antibodies against cd45 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Cytotoxic NK Cells Impede Response to Checkpoint Immunotherapy in Melanoma with an Immune-Excluded Phenotype
    Article Snippet: .. Primary antibodies against CD45 (monoclonal, rat; Novus Biologicals, cat. #NB100-77417SS; RRID: AB_1083776; 1:400) and S100 (polyclonal, rabbit; Dako, cat. #Z0311; RRID: AB_10013383; 1:200) were incubated overnight at 4°C in antibody diluent (1% BSA and 0.1% Triton-X in DPBS). .. The sections were washed in DPBS for 10 minutes and incubated with secondary antibodies (donkey antirabbit; Life Technologies, cat. #A31573, RRID: AB_2536183, 1:400 and donkey anti-rat; Thermo Fisher Scientific, cat. #A21208, RRID AB_141709) in antibody diluent for 45 minutes at room temperature.



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    Fig. 5 | Col6a1cre and Twist2cre fibroblast-specific targeting in the ileum. a H&E images of 4-µm-thick ileal paraffin sections and immunofluorescent confocal ima- ges from 12-µm-thick ileal cryosections, stained with DAPI and antibodies against Podoplanin (PDPN) and <t>CD45.</t> Images are representative of 3 mice per condtition. b, c Flow cytometry analysis of Live/Lin−/Pdpn+ ileal fibroblasts from the ileum of 2–3-month-old Twist2cre R26mT/mG (n = 7 mice/population) (b) and Col6a1cre R26mT/mG
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    Fig. 5 | Col6a1cre and Twist2cre fibroblast-specific targeting in the ileum. a H&E images of 4-µm-thick ileal paraffin sections and immunofluorescent confocal ima- ges from 12-µm-thick ileal cryosections, stained with DAPI and antibodies against Podoplanin (PDPN) and CD45. Images are representative of 3 mice per condtition. b, c Flow cytometry analysis of Live/Lin−/Pdpn+ ileal fibroblasts from the ileum of 2–3-month-old Twist2cre R26mT/mG (n = 7 mice/population) (b) and Col6a1cre R26mT/mG

    Journal: Nature communications

    Article Title: Different fibroblast subtypes propel spatially defined ileal inflammation through TNFR1 signalling in murine ileitis.

    doi: 10.1038/s41467-025-57570-7

    Figure Lengend Snippet: Fig. 5 | Col6a1cre and Twist2cre fibroblast-specific targeting in the ileum. a H&E images of 4-µm-thick ileal paraffin sections and immunofluorescent confocal ima- ges from 12-µm-thick ileal cryosections, stained with DAPI and antibodies against Podoplanin (PDPN) and CD45. Images are representative of 3 mice per condtition. b, c Flow cytometry analysis of Live/Lin−/Pdpn+ ileal fibroblasts from the ileum of 2–3-month-old Twist2cre R26mT/mG (n = 7 mice/population) (b) and Col6a1cre R26mT/mG

    Article Snippet: Tissue permeabilization was achieved with 0.03% Triton X-100 (Acros Organics, 2156825000) in PBS for 10minutes, followed by blocking in PBS containing 1% albumin for 1 h. Immunofluorescence staining on these sections involved primary antibodies against anti-CD45 (AF114, R&D Systems), Biotin-conjugated antipodoplanin (127403, Biolegend), anti-CD68 (PAS-78996, ThermoFisher), and anti-collagen IV (ab6586, Abcam).

    Techniques: Staining, Flow Cytometry